Phytochemical investigation of Croton macrostachyus crude extracts and evaluation of their antibacterial activity
Keywords:Croton macrostachyus, antibacterial activity, stem bark, root,, seed.
Croton macrostachyus is one of the most common traditional medicinal plants used in Ethiopia. The parts of this plant such as bark, twigs, fruits, leaves, roots, and seeds showed varieties of medicinal properties and cure various human and animal diseases and ailments. This paper reports the phytochemical constituents of the extracts from Croton macrostachyus plant parts grown in horo guduru wollega zone of Ethiopia and their antibacterial activities against different bacteria as well as isolation of a compound from the plant extract. Antibacterial activities of ethanol extracts of leaf, root, seed, and stem bark of Croton macrostachyus were screened against Salmonella typhimurium, Listeria monocytogenes, Staphylococcus aureus and Escherichia coli using disc diffusion method on Mueller – Hinton Agar (MHA). All the extracts showed inhibitory activity against Salmonella typhimurium, Listeria monocytogenes, and Escherichia coli. However no activity was recorded against Staphylococcus aureus. A compound, labeled as 1-18D, was isolated from the stem bark, and its structure was elucidated by 1H NMR, 13C NMR, FT-IR and compared with previously reported literature. The isolated compound showed antibacterial activity against Salmonella typhimurium and Escherichia coli, but no inhibitory activity against Listeria monocytogenes and Staphylococcus aureus. The finding of this study indicates that the ethanol extracts and the isolated compound is a promising antibacterial agent against several human pathogens.
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Copyright (c) 2021 Gedefa Dinsa , Abebe Tedla , Desalegn Yilma
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